文章基本信息

标题：EFFECTS OF RIBOFLAVIN DEFICIENCY ON LIPID PEROXIDATION OF RAT LIVER MICROSOMES
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作者：Misako TANIGUCHI
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1980
卷号：26
期号：4
页码：401-413
DOI：10.3177/jnsv.26.401
出版社：Center for Academic Publications Japan
摘要：Increases in cytochrome P-450 and cytochrome b ₅ and a decrease in NADPH-cytochrome c (P-450) reductase were generally brought about by feeding the riboflavin-deficient diet to young rats (50-120 g body weight) for 5 weeks, whereas no significant changes in these enzymes were observed with rats of 220 g body weight by feeding for 2 weeks. Amounts of lipid peroxides in the serum or the liver tissue and microsomes increased significantly after feeding the deficient diet for 5 weeks, in comparison with the respective control rats. On the other hand, NADPH-dependent lipid peroxidation in the presence of ferric ion and pyrophosphate, assayed as malondialdehyde, was decreased drastically in the liver microsomes of all groups of riboflavin-deficient rats irrespective of the period or body weight of animals. Lipid peroxidation could be detected by addition of EDTA-ferric ion or ferricyanide to the incubation medium, though the formation of malondialdehyde was less than that expected by the activity of NADPH-cytochrome c reductase. NADPH-reduction of nitroblue tetrazolium with liver microsomes was decreased in riboflavin deficiency, and was almost able to be correlated with the activity of NADPH-cytochrome c reductase. Following intraperitoneal injection of riboflavin into the deficient rats, NADPH-dependent lipid peroxidation in the presence of ferric ion and pyrophosphate recovered only to 10% of the control rates at 40 hr after the injection, when cytochrome P-450, cytochrome b ₅ and NADPH-cytochrome c reductase levels were restored to those of their respective controls. Activities of drug-metabolizing enzymes, aminopyrine demethylase and aniline hydroxylase were decreased by initiation of feeding from the weanling stage, but the activities changed only slightly by feeding from the 120g of body weight stage.
关键词：riboflavin deficiency;microsomal lipid peroxidation;microsomal enzymes;NADPH-dependent;lipid peroxidation;riboflavin-deficient rat liver enzymes;riboflavin-deficient rat liver