文章基本信息

标题：INHIBITORY EFFECTS OF Zn2+ ON MUSCLE GLYCOLYSIS AND THEIR REVERSAL BY HISTIDINE
本地全文：下载
作者：Tatsuhiko IKEDA ; Keiko KIMURA ; Sachiko MORIOKA 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1980
卷号：26
期号：4
页码：357-366
DOI：10.3177/jnsv.26.357
出版社：Center for Academic Publications Japan
摘要：Of a number of divalent cations investigated, Zn²⁺ strongly inhibited lactate production from glucose 6-phosphate in rat muscle cytosol fraction. The I₅₀ value for lactate production influenced by Zn²⁺ was 10μM and was increased to 200μM and 18μM by the addition of 10mM histidine and 10mM carnosine, respectively. The inhibitory effect of 50μM Zn²⁺ on lactate production was completely reversed by the addition of 1.2mM histidine and the apparent K _m was found to be 0.34mM. The inhibitory site for Zn²⁺ was investigated by the estimation of glycolytic intermediates. It occurred at point between fructose 6phosphate and fructose 1, 6-bisphosphate. Purified rat muscle phosphofructokinase was inhibited by Zn²⁺. The I₅₀ values for Zn²⁺ were calculated to be 12, 5.5 and 1.5μM in the presence of 3, 0.3 and 0.06mM fructose 6-phosphate, respectively. The addition of histidine removed the inhibitory effect of Zn²⁺ on the glycolytic key enzyme. These studies indicate that in rat skeletal muscle the inhibitory effect of Zn²⁺ on lactate production might result from the inhibition of phosphofructokinase and that histidine could remove the effect.
关键词：zinc;glycolysis;histidine;carnosine;glycolytic intermediates;phosphofructokinase;lactate