文章基本信息

标题：A METHOD FOR QUANTITATION OF VITAMIN B2 BY USING AN ENZYME (PRODUCING VITAMIN B2 ALDEHYDE) FROM SCHIZOPHYLLUM COMMUNE
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作者：Sei TACHIBANA ; Masanori OKA
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1980
卷号：26
期号：5
页码：419-426
DOI：10.3177/jnsv.26.419
出版社：Center for Academic Publications Japan
摘要：A method for enzymatic determinationn of riboflavin was developed by enzymatic reduction of 2, 6-dichlorophenolindophenol using a vitamin B₂-aldehyde-forming enzyme prepared from Schizophyllum commune. The enzymatic method enabled us to determine riboflavin in a mixture containing riboflavin, FMN and FAD without separation of riboflavin. Moreover, the total amount of flavins in the mixture was shown to be determined by the enzyme in the presence of phosphatase.
关键词：riboflavin;vitamin B2-aldehyde;Schizophyllum commune;2, 6 dichlorophenolindophenol;determination;vitamin B2-aldehyde-forming enzyme