文章基本信息

标题：Virus-Inactivating Effect of D-Isoascorbic Acid
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作者：Akira MURATA ; Masahiko KAWASAKI ; Hiroko MOTOMATSU 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1986
卷号：32
期号：6
页码：559-567
DOI：10.3177/jnsv.32.559
出版社：Center for Academic Publications Japan
摘要：The effect of D-isoascorbic acid, an epimer of L-ascorbic acid, on viruses was investigated using a wide variety of bacterial viruses (phages) as model systems. D-Isoascorbic acid exerted an inactivating effect on all phages examined. The reaction mechanism of virus inactiva-tion by D-isoascorbic acid was investigated using phage J 1 as a model system. Bubbling oxygen through the reaction mixture and the addition of H₂O₂ or transition metal ions into the reaction mixture enhanced the phage inactivation by D-isoascorbic acid. In contrast, nitrogen bubbling and the addition of reducing agents, chelating agents or radical scavengers prevented phage inactivation. Experiments using specific radical scaven-gers, superoxide dismutase or catalase showed that OH⋅ could be mainly responsible for phage inactivation by D-isoascorbic acid. These findings are similar to those obtained with L-ascorbic acid, and indicate that phage-inactivating activity is independent of the stereoisomerism with inversion of the hydroxyl group at carbon 5 of ascorbic acids.
关键词：D-isoascorbic acid;D-araboascorbic acid;erythorbic acid;virus inactivation;phage inactivation