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标题：The Antioxidant Effect of Palm Fruit Carotene on Skin Lipid Peroxidation in Guinea Pigs as Estimated by Chemiluminescence-HPLC Method
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作者：Keita SOMEYA ; Yuka TOTSUKA ; Michiaki MURAKOSHI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1994
卷号：40
期号：4
页码：315-324
DOI：10.3177/jnsv.40.315
出版社：Center for Academic Publications Japan
摘要：To study the antioxidant effect of palm fruit carotene on skin lipid peroxidation, the guinea pigs were orally fed ad libitum palm fruit carotene, β-carotene, or vehicle emulsions, in which carotene (0.05%, w/w) was suspended in drinking water. After treatment of carotene for 12 weeks, animals were exposed to ultraviolet ray (UV), and squalene monohydroperoxide (SqOOH)/squalene (Sq) ratios in the skin lipid were analyzed using the chemiluminescence-HPLC method. Carotene accumulation was found in the skin of guinea pigs that were orally administered palm fruit carotene or β-carotene. After UV irradiation, especially immediately after, the rise in the SqOOH/Sq ratio was effectively suppressed in both carotene-drinking groups in contrast with the control (carotene-untreated) group. An inverse correlation between the carotene content and the SqOOH/Sq ratio in the skin was also observed. The results suggested that palm fruit carotene intake prevents skin lipid peroxidation caused by UV irradiation.
关键词：palm fruit carotene;β-carotene;lipid peroxidation;squalene hydroperoxide;ultraviolet ray;antioxidant;chemiluminescence;CL-HPLC