文章基本信息

标题：Measurement of the CD3-4+ Variant T Cell Frequency by Flow Cytometry after X-Irradiation on Mice
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作者：Naoki KUNUGITA ; Nan MEI ; Satoshi NOMOTO 等
期刊名称：Journal of Occupational Health
印刷版ISSN：1341-9145
电子版ISSN：1348-9585
出版年度：1996
卷号：38
期号：1
页码：25-29
DOI：10.1539/joh.38.25
出版社：Japan Society for Occupational Health
摘要：Measurement of the CD3^-4⁺ Variant T Cell Frequency by Flow Cytometry after XIrradiation on Mice: Naoki KUNUGITA, et al. Department of Radiation Biology and Health, School of Medicine, University of Occupational and Environmental Health, Japan -Variant cell frequency of mice T-lymphocytes defective in the T-cell receptor (TCR) gene expression was measured by flow cytometry. Splenic T-lymphocytes were obtained from mice after irradiation and stained with fluorescein-labeled anti-L 3 T 4 (CD 4) and phycoerythrinlabeled anti-CD3-ε antibodies. They were analyzed with a flow cytometer to detect variant T cells lacking surface expression of TCR/CD 3 complexes which showed CD3^-4⁺ T cells. Variant T cells could be observed 3 days after 3 Gy irradiation. Frequency of variant T cells increased to the maximum level of 94×10^-4 between 15 and 21 days after 3 Gy irradiation on BALB/c mice and gradually decreased with a half-life of approximately 17 days. We examined the strain difference in radiosensitivity of splenic T-lymphocytes in BALB/c, C57BL/6, C3H/ He and B6C3F1 mouse strains by this assay method. We observed that BALB/c showed the most radiosensitivity. This assay method is quick and easy in comparison with other methods and is considered useful for the mutagenicity test. ( J Occup Health 1996; 38: 25-29 )
关键词：Strain difference;Radiosensitivity;CD3-4+ variant T-cell;T-cell receptor gene;Flow cytometry