文章基本信息

标题：PANTOTHENATE UPTAKE IN ESCHERICHIA COLI K-12
本地全文：下载
作者：Hiroshi NAKAMURA ; Zenzo TAMURA
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1973
卷号：19
期号：5
页码：389-400
DOI：10.3177/jnsv.19.389
出版社：Center for Academic Publications Japan
摘要：Characteristics of the PaA uptake process in E. coil K-12 were studied. PaA uptake was energy-, temperature-, and pH-dependent, with an optimum pH of 6.5, and was markedly inhibited by sulfuhydryl reagents (N-ethylmaleimide, monoiodoacetamide) and uncouplers of oxidative phosphorylation (pentachlorophenol, 2, 4-dinitrophenol). Saturation of PaA uptake occurred with increasing substrate concentrations. The apparent K_m was 3.0×10^-7 M. PaA was transported into the cells of E. coli without phosphorylation, and the major intracellular metabolites accumulated were CoA and DP-CoA. The results suggest the presence of an active transport system for PaA in E. coli K-12.