文章基本信息

标题：NADPH-Dependence of Vitamin B2-Aldehyde-Forming Enzyme
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作者：Sei TACHIBANA ; Masanori OKA
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1982
卷号：28
期号：4
页码：335-342
DOI：10.3177/jnsv.28.335
出版社：Center for Academic Publications Japan
摘要：Vitamin B₂-aldehyde-forming enzyme isolated from Schizo-phyllum commune with 1, 400-fold purity was found to require NADPH under aerobic but not anaerobic conditions. Both NADPH- and DCIP-requiring enzyme activities which could not be separated by our enzyme purification system were found in the same single protein band on polyacrylamide disc gel electrophoresis. The cation, Cu²⁺, markedly stimulated the NADPH-dependent enzyme reaction under the aerobic conditions. The reagents, EDTA, pyrazole, N ethylmaleimide, PCMB, and arsenite, inhibited the formation of vitamin B₂-aldehyde to various extents, whereas azide did not.
关键词：vitamin B2-aldehyde-forming enzyme;NADPH-depen-dence;DCIP-dependence;activation by Cu2+;requirement of oxygen