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标题：Immunoelectron Microscopic Localization of Sucrase-Isomaltase in Rat Small Intestine
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作者：Setsuko NODA ; Toshinao GODA
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1993
卷号：39
期号：2
页码：201-205
DOI：10.3177/jnsv.39.201
出版社：Center for Academic Publications Japan
摘要：The localization of rat small intestinal microvillous sucrase-isomaltase was studied by immunoelectron microscopy to investigate its intracellular transport to the microvillous membrane. At the same time, the usefulness of a rapid embedding method of tissues in Lowicryl K4M for immunocytochemistry of sucrase-isomaltase was examined. Sucrase-isomaltase was present not only in the microvillous membrane, but also in the apical vesicles and the apical plasma membrane invaginations. Negli-gible labeling was observed in the other portions of the absorptive cells. These findings suggest that the final step of intracellular transport of sucrase-isomaltase to the microvillous membrane is via smooth apical vesicles. The rapid immunoelectron microscopic method adopted in this study seemed to be a useful technique for the study of the intracellular localization of sucrase-isomaltase.
关键词：immunoelectron microscopic localization;sucrase-isomalt-ase;Lowicryl K4M;rat small intestine;microvillous membrne