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标题：Gross and Sepative Determination of 1α, 25-Dihydroxyvitamin D2 and D3 in Plasma Using Calf Thymus Receptor
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作者：Sonoko MASUDA ; Toshio OKANO ; Setsu MATSUOKA 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1993
卷号：39
期号：2
页码：101-114
DOI：10.3177/jnsv.39.101
出版社：Center for Academic Publications Japan
摘要：Determination of 1α, 25-dihydroxyvitamin D [1, 25(OH)₂D, gross amounts of 1, 25(OH)₂D₂ and 1, 25(OH)₂D₃] and separative deter-mination of 1, 25(OH)₂D₂ and 1, 25(OH)₂D₃ in plasma using calf thymus receptor have been investigated. A lipid extract from 1 ml of plasma is applied to a Bond Elut C₁₈OH column and an eluate corresponding to 1, 25-(OH)₂D including both 1, 25(OH)₂D₂ and 1, 25(OH)₂D₃ is applied to calf thymus receptor to assay a gross amount of the two compounds. On the other hand, when separative assay of the two compounds is performed, the 1, 25 (OH)₂D eluate obtained from the Bond Elut C₁₈OH column is further applied to HPLC using a Zorbax SIL column with 5% isopro-panol in methylene chloride as a developing solvent to separate the two compounds from one another. The separated eluates are independently applied to the receptor to assay the two compounds. Since less amounts of unknown components non-specifically bound to interfering concomi-tants besides 1, 25(OH)₂D exist in the calf thymus receptor, complicated purification steps to eliminate the concomitants are unnecessary. The detection limit by this method is 1.25 pg/tube which is sensitive enough for a routine method to assay 1, 25(OH)₂D in plasma.
关键词：vitamin D;1,25-dihydroxyvitamin D;1,25-dihydroxyvitamin D2;1,25-dihydroxyvitamin D3;radioreceptor assay;calf thymus receptor