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标题：Purification and Properties of Branched Chain Amino Acid Aminotransferase from Gramicidin S-Producing Bacillus brevis
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作者：Masayuki KANDA ; Kazuko HORI ; Toshitsugu KUROTSU 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1995
卷号：41
期号：1
页码：51-60
DOI：10.3177/jnsv.41.51
出版社：Center for Academic Publications Japan
摘要：The branched chain amino acid aminotransferase [EC 2.6.1.42] was purified to a homogeneous state from a gramicidin S-producing strain of Bacillus brevis . The enzyme had a molecular weight of about 93, 000 and consisted of two identical subunits, each with a molecular weight of about 47, 000. One pyridoxal phosphate is bound per subunit. In addition to branched chain amino acids, the enzyme uses L-phenylalanine and L-tryptophan as the amino donor, indicating that B. brevis branched chain amino acid aminotransferase has a broad substrate specificity for the amino donor. The enzyme utilized 2-oxoglutarate as the amino acceptor. The purified enzyme exhibits its absorption maxima at 332 and 427 nm at neutral pH.
关键词：branched chain amino acid aminotransferase;Bacillus brevis;Gramicidin S;pyridoxal phosphate