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标题：Isolation, Identification and Biological Activity of 24R, 25-Dihydroxy-3-epi vitamin D3: A Novel Metabolite of 24R, 25-Dihydroxyvitamin D3 Produced in Rat Osteosarcoma Cells (UMR 106)
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作者：Maya KAMAO ; Syuichiro TATEMATSU ; G Satyanarayana REDDY 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2001
卷号：47
期号：2
页码：108-115
DOI：10.3177/jnsv.47.108
出版社：Center for Academic Publications Japan
摘要：We recently identified 1α, 25-dihydroxy-3-epi-vitamin D₃ [1 α, 25(OH)₂-3-epi-D₃] as a metabolite of 1 α, 25-dihydroxyvitamin D₃ [1α, 25(OH)₂D₃] produced in rat osteosarcoma cells (UMR 106). We now report the isolation of 24 R , 25-dihydroxy-3-epi-vitamin D₃ [24 R , 25 (OH)₂-3-epi-D₃] as a metabolite of 24 R , 25-dihydroxyvitamin D₃ [24 R , 25(OH)₂D₃] by high-performance liquid chromatography (HPLC) with chiral column and its structure assignment by proton nuclear magnetic resonance (¹H-NMR) and liquid chromatography-mass spectrometry (LC-MS) analysis. We also demonstrated the production of 24 R , 25(OH)₂-3-epi-D₃ in two other cell lines [human colon carcinoma cells (Caco-2) and porcine kidney cells (LLC-PK₁)] which were previously shown to convert 1 α, 25(OH)₂D₃ into 1 α, 25 (OH)₂-3-epi-D₃. It can be seen that the production of 24 R , 25 (OH)₂-3-epi-D₃ from 24 R , 25(OH)₂D₃ is lower than that of 1 α, 25 (OH)₂-3-epi-D₃ from 1α, 25(OH)₂D₃ in all the cells studied. 24 R , 25(OH)₂-3-epi-D₃ was found to be inactive in terms of its ability to bind to the vitamin D receptor (UDR), in inhibiting proliferation and in inducing differentiation of human promyelocytic leukemia cells (HL-60). Thus, our study indicates that the C-3 epimerization pathway is common to both lα, 25(OH)₂D₃ and 24 R , 25(OH)₂D₃ and may play an important role in modulating the concentration and the biological activity of these two major vitamin D₃ metabolites in target tissues.
关键词：24R,25(OH)2D3;24R,25(OH)2-3-epi-D3;1α,25(OH)2D3;1α,25(OH)2-3-epi-D3;metabolism