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标题：Primary Screening of Relatively Less Allergenic Wheat Varieties
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作者：Akihiro NAKAMURA ; Soichi TANABE ; Jun WATANABE 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2005
卷号：51
期号：3
页码：204-206
DOI：10.3177/jnsv.51.204
出版社：Center for Academic Publications Japan
摘要：Since the number of wheat-allergic patients has been increasing in recent years, less allergenic wheat varieties are socially demanded. The aim of this study was to primarily screen 324 varieties of wheat picked up from various parts of the world. Prior to the screening, the inhibition ELISA (enzyme-linked immunosorbent assay) was examined with major wheat allergens (glutenin, gliadin and α-amylase inhibitor). As a result, the patient's IgE antibodies used in this assay reacted to these three allergens, and thereby turned out to be suitable for the primary screening for the less allergenic wheat varieties. Thus, the aller-genicity of each wheat sample was measured by direct ELISA using the sole patient's serum as an IgE source and ‘Aoba Komugi’ as a control (a value of 1.0). The mean±SD of all kinds was 1.14±0.37. Among them, ‘CM32859’ (Mexico) gave the lowest value (0.38). Several other varieties, such as ‘Einkorn’ (Unknown), ‘Iliniaza’ (Ecuador), ‘Hsin Mai 2’ (China), ‘Italy 1’ (Italy) and so on, also serve as candidates for the less allergenic varieties. More detailed secondary screening will lead to a finding of the less allergenic wheat varieties for wheat-allergic patients, because these varieties are expected to become mother plants for breeding.
关键词：wheat allergen;ELISA;less allergenic variety