文章基本信息

标题：THE EFFECT OF 1α-HYDROXYVITAMIN D3 AND CORTISONE ON THE DEVELOPMENT OF CHICK DUODENAL ALKALINE PHOSPHATASE IN ORGAN CULTURE
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作者：Setsuko YOSHIZAWA ; Noriko SUGISAKI ; Sachiko MORIUCHI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1976
卷号：22
期号：1
页码：21-28
DOI：10.3177/jnsv.22.21
出版社：Center for Academic Publications Japan
摘要：The development of alkaline phosphatase influenced by 1α-OH-D₃ (a synthetic active form of vitamin D₃) and cortisone was studied in chick duodenal organ cultures. The administration of cortisone to the embryo in ovo on the 14th day of incubation resulted in a precocious increase in alkaline phosphatase after 6 days (20-day embryo). When duodena from 14-, 18- and 20-day embryos were cultured in the presence of cortisone, there was no significant enhancement of alkaline phosphatase activity except for a marginal effect observed in the 18-day duodenum. On the other hand, the alkaline phosphatase activity in cultured duodena from 20-day chick embryos was significantly stimulated by the addition of 1α-OH-D₃. The effects of cortisone and 1α-OH-D₃ were not additive. The activity of maltase, another intestinal enzyme, was not influenced by 1α-OH-D₃. Studies on inactivation of alkaline phosphatase by EDTA suggest that the observed increase in alkaline phosphatase activity induced by the administration of 1α-OH-D₃ in vitro may be related to the qualitative changes in the enzyme that take place during development in vivo .