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标题：LEVEL OF METHIONINE SYNTHASE ACTIVITY AND INTERCONVERSION OF METHYLCOBALAMIN AND ADENOSYLCOBALAMIN IN A FACULTATIVE METHYLOTROPH, PROTAMINOBACTER RUBER
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作者：Kazuyoshi SATO ; Shoichi SHIMIZU
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1980
卷号：26
期号：6
页码：557-569
DOI：10.3177/jnsv.26.557
出版社：Center for Academic Publications Japan
摘要：Protaminobacter ruber was cultured in a medium containing [⁵⁷Co]cyanocobalamin with a “two-step cultivation method” and the forms of vitamin B₁₂ compounds in the cells were examined. Methyl cobalamin was detected in the early phases of growth and reached a maximum of about 40% of ail cobalamins extracted from the cells. In the stationary phase of growth, almost all cobalamins consisted of ade nosylcobalamin. Recultivation of the cells of the stationary phase in a fresh medium resulted in the conversion of adenosylcobalamin into methylcobalamin. Interconversion of methylcobalamin and adenosyl cobalamin was presumed from these facts. The formation of adenosylcobalamin from methylcobalamin was de monstrated with a cell-free extract system from P. ruber. The rate of conversion of methylcobalamin into adenosylcobalamin was highest among several cobalamin analogs tested. Propylation of 5-methyltetrahydrofolate: homocysteine methyltransferase with 1-iodopropane did not affect this conversion reaction, which was probably catalyzed by methyltransferase and adenosyltransferase.
关键词：Protaminobacter ruber;facultative methylotroph;methionine synthase;methylcobalamin;adenosylcobalamin;vitamin B12 interconversion