文章基本信息

标题：Mechanism of Uptake of Cobalamin into Enterocyte Mitochondria
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作者：Masami INADA ; Masanori TOYOSHIMA
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1982
卷号：28
期号：3
页码：247-254
DOI：10.3177/jnsv.28.247
出版社：Center for Academic Publications Japan
摘要：To study the mechanism which cobalamin is taken up by the mitochondria, ⁵⁷Co-cyanocobalamin-binder complex and freshly pre-pared mitochondria were prepared from the enterocytes of rats. Subsequently, the binder complex was incubated together with mitochon-dria in a calcium-containing medium in vitro . Uptake of cobalamin was determined by measuring the radioactivities bound to the mitochondria. Consequently, lysosomal and microsomal binders enhanced cobalamin uptake into the mitochondria, but intrinsic factor did not. It was found that the uptake into the mitochondria was inhibited by previous treatment with calcium-chelating agents. The uptake was com-pletely restored after addition of calcium ions to the mitochondria.
关键词：uptake mechanism;cobalamin;intracellular binder;lyso-somal binder;mitochondria;transcobalamin II;intrinsic factor;rat enterocytes;calcium ions;calcium-chelating agent