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标题：Hydrolysis and Synthesis of Thiamin Triphosphate in Bacteria
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作者：Takahiro NISHIMUNE ; Ryoji HAYASHI
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1987
卷号：33
期号：2
页码：113-127
DOI：10.3177/jnsv.33.113
出版社：Center for Academic Publications Japan
摘要：Thiamin triphosphate (ThTP) in early stationary phase cells of Escherichia coli grown in nutrient broth with 0.1% yeast extract was found to constitute approximately 5-7% of cellular thiamin diphosphate (ThDP) or around 5 nmol/g cell. Nearly the same level of. ThTP was obtained in a Bacillus strain. When E. coli was loaded with an excess of ThTP or ThDP, cellular ThTP was found to be controlled in the course of the long term to maintain its ratio to the amount of cellular ThDP. The ThTP vs . ThDP ratio in E. coli cells after short-term ThDP uptake was found to be a function of the cellular growth phase. The ratio in early exponential phase E. coli cells was found to be approximately 4% and it became lower (less than 3%) when cell growth proceeded to the late exponential stage. Two phosphatases specific for ThTP (ThTPase) among thiamin phosphates were detected in E. coll. One required Mg²⁺ and was found mainly in the soluble fraction, while the other was Mgt²⁺independent and originated from the membrane. The two ThTPases were similar to their rat tissue counterparts.
关键词：thiamin triphosphate;thiamin triphosphatase;thiamin diphosphate kinase;thiamin pyrophosphate;thiamin;E. coli