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标题：Effect of Branched-Chain Amino Acid on 15N Incorporation into Liver and Skeletal Muscle Proteins Following [15N]-Ammonium Chloride Administration to Carbon Tetrachloride-Intoxicated Rats
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作者：Misako OKITA ; Akiharu WATANABE ; Takao TSUJI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1988
卷号：34
期号：1
页码：85-96
DOI：10.3177/jnsv.34.85
出版社：Center for Academic Publications Japan
摘要：The effect of branched-chain amino acid (BCAA) on protein synthesis and nitrogen metabolism in liver and skeletal muscle was evaluated by an intraperitoneal injection of [¹⁵N]ammonium chloride (¹⁵NH₄Cl) to carbon tetrachloride (CCl₄)-intoxicated rats. The ¹⁵NH₄Cl was bolusly injected at a dose of 6mg/100g body weight one hour after an amino acid solution containing leucine and valine (150mM each, abbre-viated as BCAA), phenylalanine and alanine (150mM each, PA), 120mM leucine and 30mM valine (Leu-rich) or 120mM valine and 30mM leucine (Val-rich) was administered intragastrically at a dose of 2.5ml each 100g body weight. The ¹⁵N-enrichment in the protein fraction of the liver was higher in CCl₄-BCAA group than CCl₄-PA group. The Leu-rich solution was found more effective in enhanced incorporation of ¹⁵N into liver and skeletal muscle proteins. The disappearance rate of [¹⁵N]urea from the plasma, which was influenced by the synthesis from ¹⁵NH₃ and the excretion into urine, was much faster in the CCl₄-BCAA group than CCl₄-PA group. In Leu-rich group, both ¹⁵N incorporation into non-protein fraction of skeletal muscle and disappearance rate of plasma urea-1¹⁵N were greater than those in Val-rich group. The results suggest that BCAA, particularly leucine, has beneficial effects on protein synthesis and am-monium detoxification in liver-injured rats.
关键词：branched-chain amino acid;nitrogen-15;[15N]ammonium chloride;protein synthesis;liver-injured rat