文章基本信息

标题：Suppression of Pentylenetetrazol-Induced Seizures and c-fos Expression in Mouse Brain by L-Carnitine
本地全文：下载
作者：Yoshihisa IRYO ; Masato MATSUOKA ; Hideki IGISU 等
期刊名称：Journal of Occupational Health
印刷版ISSN：1341-9145
电子版ISSN：1348-9585
出版年度：2000
卷号：42
期号：3
页码：119-123
DOI：10.1539/joh.42.119
出版社：Japan Society for Occupational Health
摘要：Suppression of PentylenetetrazolInduced Seizures and c- fos Expression in Mouse Brain by L-Carnitine: Yoshihisa IRYO, et al . Department of Environmental Toxicology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health —Seizure development in ddY mice was recorded on a videotape for 20 min after injection of pentylenetetrazol (PTZ) (50 mg/kg) and analyzed in detail. In addition, expression of the c- fos gene in the brain was semi-quantified by reverse transcription-polymerase chain reaction analysis. When saline, L-carnitine (10 mmol/kg), or Dcarnitine (10 mmol/kg) was administered 30 min prior to PTZ, the seizures were suppressed in mice given Lcarnitine; the seizure scores were significantly lower in mice treated with L-carnitine at several time points, and the overall seizure scores were 31.43 ± 2.49, 10.57 ± 2.86 and 24.71 ± 3.05 (expressed as the mean ± S.E.M., n=7), in saline, L-carnitine and D-carnitine groups, respectively. The latency to the first clonictonic seizure was also prolonged in mice treated with L-carnitine. The level of c- fos mRNA in the brain was lower in the animals treated with L-carnitine than in those treated with saline or D-carnitine. Thus, Lcarnitine shows not only anticonvulsive effects in one of the most widely used animal models of chemically induced seizures but also the potential to suppress the seizure-associated expression of an immediate early gene in the brain.
关键词：Seizures;Carnitine;Pentylenetetrazol;Immediate early genes;c-fos