文章基本信息

标题：Effect of Retinoids on Proliferation of Human Embryonic Palatal Mesenchymal Cells in Culture
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作者：Toshiaki WATANABE ; William D. WILLIS ; Robert M. PRATT 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1990
卷号：36
期号：4-SupplementI
页码：311-325
DOI：10.3177/jnsv.36.4-SupplementI_311
出版社：Center for Academic Publications Japan
摘要：The cytokinetics and growth effects of retinnic acid (RA) (13- cis -RA, and all- trans -RA) were determined in human embryonic palatal mesenchymal (HEPM) cells using cell culture and sister chromatid differential staining. The lowest concentration tested that showed growth inhibition was 1.0×10^-4 M for 13- cis -RA, and 6.8×10^-5 M for all- trans -RA after 40 h of treatment. When HEPM cells were grown in the presence of BrdU (5-bromodeoxyuridine) for 44 h, approximately 70% of the control cells were in metaphase in the 3rd replication cycle (M3). The percentage of cells in M3 decreased in the presence of RA with or without a metabolic activation system (S-9), thus indicating a longer cell proliferation time for the RA-treated cells. The estimate of cell proliferation time in 13- cis -RA-treated cells (1.0×10^-4 M) was approximately 25 h at the 2nd cell cycle compared with 20 h in the control cells. When HEPM cells were exposed for 16 h to [³H] -thymidine, labeling indices in 13- cis -RA-treated cells were significantly reduced even at 1.0×10^-6 M. Both 13- cis -RA and all- trans -RA caused concentration-dependent decreases in [³H] -thymidine incorporation into HEPM cells. These results suggest that retinoic acids or their major metabolites interfere with DNA synthesis and decrease the proliferation of HEPM cells.
关键词：retinoic acids;embryonic mesenchymal cells;HEPM;cell culture;sister chromatid differentiation (SCD);cell proliferation;cytokinetics;DNA synthesis;mitotic index