文章基本信息

标题：Simple Method of Tocopherol Determination in Plasma Lipoprotein Fractions Using a Heparin-Ca Precipitation Technique
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作者：Makoto KITAGAWA ; Toshie MORITA ; Makoto MINO 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1989
卷号：35
期号：4
页码：235-242
DOI：10.3177/jnsv.35.235
出版社：Center for Academic Publications Japan
摘要：A simple and convenient method using a heparin-Ca precipitation technique, which was devised for determination of serum lipoprotein fractions, was applied to the determination of tocopherol concentrations in individual lipoproteins. Three types of precipitates were prepared from 0.25ml serum samples plus 0.1 ml of 5% heparin by mixing at 37°C for 30 min with 5 ml of 0.054 M CaCl₂ only, 0.054 M CaCl₂ in 0.6 NaCl, or 0.054 M CaCl₂ in 0.92% NaCl. These three precipitates consisted of chylomicron+VLDL (very low-density lipoprotein)+LDL (low density lipoprotein), chylomicron+VLDL, and chylomicron only, respectively. The tocopherol content of individual lipoproteins was calculated from the concentrations of tocopherol in these three precipitates, and the values obtained by using this technique were very close to those by a standard ultracentrifugation method. Thus, this simple method appears to be suitable for rapid tocopherol assay of a large number of samples.
关键词：vitamin E;lipoproteins;serum;precipitation technique