文章基本信息

标题：Metabolism of Arachidonic, Eicosapentaenoic, and Docosahexaenoic Acids in HepG2 Cells and Rat Hepatocytes
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作者：Yoko FUJIYAMA-FUJIWARA ; Rumi UMEDA ; Osamu IGARASHI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1992
卷号：38
期号：4
页码：329-334
DOI：10.3177/jnsv.38.329
出版社：Center for Academic Publications Japan
摘要：The metabolism of arachidonic acid (AA), eicosapenta-enoic acid (EPA), and docosahexaenoic acid (DHA) was examined in HepG2 cells, a human hepatoma cell line, and rat hepatocytes. The AA level in HepG2 cells was lower than in rat hepatocytes and incorporation of AA into HepG2 was also smaller than into rat hepatocytes. Both cells could not increase the level of cellular DHA by the addition of exogenous 22:5 ( n -3); whereas, rat hepatocytes, but not HepG2 cells, increased the levels of AA from 20:3 ( n -6) and EPA from 20:4 ( n -3). In both cells, retroconversion of AA to 20:3 ( n -6) occurred, but EPA was not retro-converted to 20:4 ( n -3). These results suggested that the levels of AA and DHA in both types of cells, were regulated more severely than EPA and that the activity of fatty acid desaturation might be different between n -6 and n -3 families.
关键词：HepG2;rat hepatocyte;arachidonic acid;eicosapentaenoic acid;docosahexaenoic acid;desaturation;retroconversion