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标题：Characterization of Thiamin-Binding Protein from Buckwheat Seeds
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作者：Katsumi WATANABE ; Mayumi SHIMIZU ; Takashi ADACHI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1998
卷号：44
期号：2
页码：323-328
DOI：10.3177/jnsv.44.323
出版社：Center for Academic Publications Japan
摘要：A thiamin-binding protein from buckwheat ( Fagopyrum esculentum Moench) seeds gave two bands of 56- and 50-kDa in the absence of 2-mercaptoethanol and a single band of 25-kDa in the presence of 2-mercaptoethanol on sodium dodecylsulfate gel electrophoresis. These results indicate that the protein consists of polypeptides linked by disulfide bond(s). The protein isolated from buckwheat seeds did not have immunological homology with the thiamin-binding proteins from rice seeds and sesame seeds. However, the binding of the protein to thiamin was inhibited by the modification of the carboxyl residues in the protein as well as that of the thiamin-binding protein from rice seeds. These results suggest that the thiamin-binding protein from buckwheat seeds differs from those from rice seeds and sesame seeds as to subunit structure or immunological properties, but resembles them in the mechanism of binding thiamin.
关键词：buckwheat;seed protein;thiamin;thiamin-binding activity;thiamin-binding protein