文章基本信息

标题：Evidence for Other Binding Component (s) than Ratinoid Receptors Specifically Interacting with a Retinoic Acid Response Element in the Rat Liver
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作者：Shigeaki KATO ; Hikaru MORINO ; Hiroshi MANO 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1994
卷号：40
期号：1
页码：1-10
DOI：10.3177/jnsv.40.1
出版社：Center for Academic Publications Japan
摘要：A nuclear component interacting with a retinoic acid re-sponse element (RARE-β) derived from the all- trans -retinoic acid recep-tor β (RARβ) gene promoter was detected in the rat liver by gel-shift assay. Competition experiments using the competitors of the other retinoid response elements and non-specific sequences upon the binding of this hepatic component (HBC) to the RARE-β revealed that the binding of the HBC to the RARE-β was competed only by the self competitor, but not the other elements, suggesting that the HBC is specific for the RARE-β. Moreover, although the specific monoclonal antibodies for RARα, RARβ, and RARγ reacted and shifted up the DNA complexes of endogenous as well as recombinant RARs, the DNA complex with HBC was not subjected to the immunoreaction with the antibodies. Thus, newly identified HBC, which is distinct from the characterized retinoid receptors, may modulate retinoid signaling through its direct binding upon the RARE-β.
关键词：vitamin A;gene expression;retinoid response elements;retinoid receptors;nuclear factor;rat liver