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标题：Content and Characteristics of Vitamin B12 in Some Seaweeds
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作者：Shoji YAMADA ; Yoshie SHIBATA ; Mitsue TAKAYAMA 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1996
卷号：42
期号：6
页码：497-505
DOI：10.3177/jnsv.42.497
出版社：Center for Academic Publications Japan
摘要：The vitamin B₁₂ (B₁₂) content in seven species of seaweed that are consumed frequently in Hokkaido, Japan, was microbiologically measured using Escherichia coil 215. Asakusanori ( Porphyra tenera ), maruba-amanori ( Porphyra suborbiculata ) and akaba-gin-nansou ( Rhodogtossum pulcherum ) showed higher B₁₂ content than the other species, although the content varied greatly among samples in the same species. A bioautography on a thin-layer plate holding a mixture of silica gel and cellulose, differentiation of B₁₂ and its analogues using a binding specificity of intrinsic factor and haptocorrin, and comparison of the B₁₂ concentra tion determined by the radioisotope dilution assay method using the intrinsic factor as the B₁₂-binding protein with that by the bioassay method, predominantly showed B₁₂ in maruba-amanori and B₁₂ analogues in wakame (Undaria pinnatifida) and akaba-gin-nansou. The B₁₂ uptake of akaba-gin-nansou from artificial seawater was similar to that of asaku sanori that contained only B₁₂.
关键词：vitamin B12;seaweed;B12 analogue;bioassay;radioassay;B12 uptake