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标题：Pre-column Derivatization and Its Optimum Conditions for Quantitative Determination of Urinary Hydroxylysine Glycosides by High-Performance Liquid Chromatography
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作者：Kazuhiro YOSHIHARA ; Naritoshi MOCHIDOME ; Yasuo SHIDA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：6
页码：604-607
DOI：10.1248/bpb.16.604
出版社：The Pharmaceutical Society of Japan
摘要：A method has been developed for rapid analysis of the urinary hydroxylysine glycosides [glucosyl-galactosyl-hydroxylysine (GGH) and galactosyl-hydroxylysine (GH)] as collagen metabolites. The glycosides were fluorometrically derivatized with 5-dimethylaminonaphthalene-1-sulfonyl chloride, and then subjected to reversed-phase high-performance liquid chromatography using a gradient system. Suitable standard hydroxylysine glycosides which were not diastereoisomers were prepared from human urine. Urinary GGH and GH were analyzed within 1 h, including column washing. This method was applied to the urine samples of 68 healthy persons of different ages.
关键词：hydroxylysine glycoside;fluorescence derivatization;collagen degradation;urinary excretion level;HPLC;electrospray ionization mass spectrometry