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标题：Effect of Divalent Metal Ions on the Binding of Thyroxine to Bovine Serum Albumin as Measured by Fluorescence
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作者：Nobuo OKABE ; Misa HOKAZE
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：7
页码：719-721
DOI：10.1248/bpb.16.719
出版社：The Pharmaceutical Society of Japan
摘要：The binding of thyroxine (T₄) to bovine serum albumin (BSA) has been studied in the presence and absence of Ca²⁺, Cu²⁺ and Zn²⁺ ions at various pH's in 0.1 M Tris-acetate buffer at 25°C using the fluoresence method. In the presence of 50 μM Ca²⁺ and Zn²⁺ and the absence of metal ions, the binding constant (K) increased similarly with increasing pH values from pH 5 to pH 9, and the K value near midpoint, pH 7.4, was 1.66±0.17×10⁶ M^-1. By contrast, the binding constant remained constant between pH 5 and pH 9 in the presence of 10 μM Cu²⁺, with an average value of 1.61±0.22×10⁶ M^-1, suggesting a significant influence of Cu²⁺ ions on T₄ binding to BSA.
关键词：thyroid hormone;thyroxine;bovine serum albumin;binding constant;metal ion