文章基本信息

标题：Determination of the Molecular-Weight Distribution of Low-Molecular-Weight Heparins Using High-Performance Gel Permeation Chromatography
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作者：Hiroaki KOMATSU ; Tomoko TAKAHATA ; Masakazu TANAKA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：12
页码：1189-1193
DOI：10.1248/bpb.16.1189
出版社：The Pharmaceutical Society of Japan
摘要：The molecular-weight distribution of commercially available low-molecular-weight heparins (LMWHs) was estimated by high-performance gel permeation chromatography (HPGPC) using a LMWH molecular-weight calibrant, supplied by the National Institute for Biological Standards and Control for the calibration of columns. This calibrant is a mixture of many polysaccharides with a disaccharide unit prepared by a random depolymerization of unfractionated heparin, and the ends of the fragments have a UV absorption. The ratio of the refractive index to the UV absorption is a relative measure of molecular weight. Various molecular-weight parameters such as weight-, number- or z-average molecular-weights, polydispersity and relative distributions of high, middle and low molecular-weight components were estimated, indicating that LMWHs have a wide variation in molecuar-weight distribution. This variation was ascribed to the difference in preparation methods from unfractionated heparins.
关键词：low-molecular-weight heparin;molecuar-weight distribution;heparin;high-performance gel permeation chromatography;polysaccharide