文章基本信息

标题：Effect of Guanidine Hydrochloride on the Conformation of Egg Yolk Low Density Lipoprotein
本地全文：下载
作者：Nobuo OKABE ; Yumi YOSHII
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1994
卷号：17
期号：6
页码：853-855
DOI：10.1248/bpb.17.853
出版社：The Pharmaceutical Society of Japan
摘要：The denaturation of egg yolk low-density lipoprotein (LDL) induced by guanidine hydrochloride (GdnHCl) has been investigated by using circular dichroism (CD) and fluorescence measurements. The transition curves were obtained from changes of the residue ellipticity at 222 nm and the fluorescence intensity of the aromatic residues, tyrosine and fluorescence. Different transition curves were obtained at the molarities of GdnHCl between 2 and 6 M, and between 1 and 4 M from the CD and the fluorescence measurements, respectively. The noncoincidence of the transition curves as well as the asymmetry of the transition curve obtained from the fluorescence measurements suggests that the denaturation process of LDL consists of several transition states. A very low apparent free energy of denaturation (ΔG^H₂O_app=3.0 kcalmol^-1) obtained by CD data suggests that LDL has an unstable folded structure in its native state.
关键词：egg yolk low-density;lipoprotein;denaturation;guanidine hydrochloride;circular dichroism;fluorescence