文章基本信息

标题：MOLECULAR CLONING OF cDNA ENCODING RAT 2, 3-OXIDOSQUALENE : LANOSTEROL CYCLASE
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作者：Masayuki KUSANO ; Masaaki SHIBUYA ; Ushio SANKAWA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1995
卷号：18
期号：1
页码：195-197
DOI：10.1248/bpb.18.195
出版社：The Pharmaceutical Society of Japan
摘要：A cDNA encoding rat 2, 3-oxidosqualene : lanosterol cyclase, the enzyme responsible for the complex cyclization/rearrangement step in sterol biosynthesis, was cloned by extensive application of PCRs. Oligonucleotide primers were designed in sense and anti-sense directions corresponding to internal peptide sequences of purified enzyme. Successive PCRs with all possible combinations of these primers yielded a 178-bp fragment, and based on its sequence full nucleotide sequence of cDNA was obtained by a "rapid amplification of cDNA ends"(RACE) method.
关键词：2, 3-oxidosqualene;lanosterol;cyclase;cDNA;PCR;rat liver