文章基本信息

标题：Further Metabolism of 2-Formylaminofluorene, a Metabolite of 2-Aminofluorene, in Rats
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作者：Osamu UEDA ; Shigeyuki KITAMURA ; Kiyoshi TATSUMI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：2
页码：330-333
DOI：10.1248/bpb.19.330
出版社：The Pharmaceutical Society of Japan
摘要：The further in vivo metabolism of 2-formylaminofluorene (FAF), which is a metabolite of 2-aminofluorene, was examined in rats. The 48 h urine collected following oral administration of FAF to rats was treated with β-glucuronidase/arylsulfatase and extracted with benzene. When the benzene extract was subjected to HPLC, ten peaks due to FAF and its metabolites were detected. Among these metabolites, major metabolites 1, 2, 4 and 5 were isolated and identified unequivocally as 7-hydroxy-2-formylaminofluorene, 7-hydroxy-2-acetylaminofluorene, 5-hydroxy-2-formylaminofluorene and 5-hydroxy-2-acetylaminofluorene, respectively, by comparing their MS and UV spectra, and HPLC and TLC behavior with that of authentic specimens. Minor metabolites, 7-hydroxy-2-aminofluorene, 5-hydroxy-2-aminofluorene, 2-acetylaminofluorene and 2-aminofluorene together with the parent compound were detected by HPLC. These metabolites of FAF were also detected in the feces of rats given FAF.
关键词：2-formylaminofluorene;in vivo metabolism;7-hydroxy-2-formylaminofluorene;7-hydroxy-2-acetylamino-fluorene;5-hydroxy-2-formylaminofluorene;5-hydroxy-2-acetylaminofluorene