文章基本信息

标题：Influence of Aging on Rolipram-Sensitive Phosphodiesterase Activity and [3H] Rolipram Binding in the Rat Brain
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作者：Michihisa TOHDA ; Toshihiko MURAYAMA ; Shinsuke NOGIRI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：2
页码：300-302
DOI：10.1248/bpb.19.300
出版社：The Pharmaceutical Society of Japan
摘要：To clarify the quantitative and qualitative changes in type IV phosphodiesterase (PDE IV) with aging, phosphodiesterase (PDE) activity and [³H] rolipram binding in the cytosolic fraction from the brains of young and aged rats were examined. In all areas of the aged (100-week-old) rat brain except for hippocampus, the PDE activity was decreased by about half that in the young (10-week-old) animals. However, inhibition % by 100μM rolipram and by 100μM isobutylmethylxanthine (IBMX) was not significantly different between the aged and young rats. On the other hand, [³H] rolipram binding and rolipram-sensitive PDE activity did not change with aging in the hippocampus, although both were decreased in other brain regions. These results suggest that PDE IV does not decrease with aging and maintains its cAMP degrading activity in the hippocampus. It may be involved in the dysfunction of the hippocampus with aging.
关键词：phosphodiesterase;rolipram;aging;hippocampus