文章基本信息

标题：A New Method for Permeabilization of the Plasma Membrane of Cultured Mammalian Cells. II. Mechanism for Potentiation of Bleomycin Cytotoxicity by Vortex-Stirring with High Molecular Weight Polyacrylic Acid
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作者：Naoki SHIMIZU ; Yutaka KAWAZOE
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：6
页码：805-808
DOI：10.1248/bpb.19.805
出版社：The Pharmaceutical Society of Japan
摘要：As previously reported, the cytotoxicity of bleomycin (BLM) toward cultured mammalian cells was remarkably potentiated by vortex-stirring this agent with the cells for a few seconds in the presence of high molecular weight polyacrylic acid (PAA). This paper describes that the enhancement of BLM cytotoxicity was also achieved by a sequential BLM-treatment immediately after vortex-stirring cells with PAA only. It was suggested that this enhancement resulted from the transient permeabilization of the plasma membrane induced during vortex-stirring in the presence of PAA, leading to an increase in BLM incorporation. This mechanism was supported by the finding of an appreciable uptake by vortex-stirred cells of Lucifer Yellow, a known non-permeant material. This procedure, which did not affect any cell viability, might be a beneficial method for transient permeabilization of cultured mammalian cells to facilitate the internalization of non-permeant materials.
关键词：permeabilization;polyacrylic acid;bleomycin;plasma membrane