文章基本信息

标题：Free Radicals from 1-Palmitoyl-2-arachidonoylphosphatidylcholine Liposomes in Fe2+/Ascorbic Acid Solution
本地全文：下载
作者：Tomokazu YOSHIDA ; Hiroshi OTAKE ; Yukihiko ARAMAKI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：6
页码：779-782
DOI：10.1248/bpb.19.779
出版社：The Pharmaceutical Society of Japan
摘要：The generation of free radicals during the lipid peroxidation of liposomes composed of 1-palmitoyl-2-arachidonoylphosphatidylcholine (PAPC-liposome) in Fe²⁺/ascorbic acid (AsA) solution was studied by the ESR spin trapping technique. A carbon-centered radical adduct was observed using α-(4-pyridyl-1-oxide)-N-tert-butyl-nitorone (4-POBN) and 5, 5-dimethyl-1-pyrroline-N-oxide (DMPO), but no oxygen-centered radicals such as ·OH, LO·, and LOO· were observed. The lipid peroxidation evaluated as 2-thiobarbituric acid reactive substances was inhibited by the addition of 4-POBN. The intensity of this inhibitory effect was dependent on the time when 4-POBN was added to the mixture of PAPC-liposomes and Fe²⁺/AsA solution, and no inhibitory effect could be observed after 4 min. The signal intensity of the carbon-centered radical adduct was dependent on the lipid concentration of PAPC-liposomes. These results suggest that the alkyl radicals generated from PAPC-liposome peroxidation induced by Fe²⁺/AsA were trapped by DMPO or 4-POBN at an earlier stage of lipid peroxidation.
关键词：free radical;PAPC-liposome;ferrous ion/ascorbic acid solution;spin trapping;ESR