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标题：Ascorbic Acid 2-O-α-Glucoside, a Stable Form of Ascorbic Acid, Rescues Human Keratinocyte Cell Line, SCC, from Cytotoxicity of Ultraviolet Light B
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作者：Eriko MIYAI ; Mitsuhiro YANAGIDA ; Jun-ichi AKIYAMA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：7
页码：984-987
DOI：10.1248/bpb.19.984
出版社：The Pharmaceutical Society of Japan
摘要：Environmental exposure to ultraviolet light B (UVB, wave lengths 290-320 nm) of the solar spectrum causes major damage, including an inflammatory response, in skin. In the present study, we estimated the ability of a stable derivative of ascorbic acid, ascorbic acid 2-O-α-glucoside (AA-2G), to reduce UVB damage, using the human keratinocyte cell line, SCC, established from squamous cell carcinoma. By pre- (9 h) and post-cultivation with AA-2G, a significant preventive effect on the decrease in the absolute number of surviving cells by exposure to UVB (typical dose, 20 mJ/cm²) was measurable by a neutral red-uptake assay. The release of leactate dehydrogenase from the cell membrane damaged by UVB was inhibited by AA-2G. In agarose gel electrophoresis, relatively high molecular weight DNA fragments were detected in irradiated cells after 6 h post-irradiation, suggesting that the mechanism of cell death was necrosis. Quantitative analysis of DNA content by flow cytometry indicated that AA-2G suppressed both an increase in debris with degraded nuclei and a decrease in cells in G1 and S phases, but not in the G2/M phase, by UVB exposure. These data suggest that AA-2G shows a photoprotective effect against UVB-induced damage in human epithelial cells.
关键词：ascorbic acid 2-O-α-glucoside;UVB;photoprotective effect;human keratinocyte cell line;SCC