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标题：Purification and Characterization of α-L-Rhamnosidase from Bacteroides JY-6, a Human Intestinal Bacterium
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作者：Il-Sung JANG ; Dong-Hyun KIM
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：12
页码：1546-1549
DOI：10.1248/bpb.19.1546
出版社：The Pharmaceutical Society of Japan
摘要：An α-L-rhamnosidase (EC 3.2.1.40) was purified 1500-fold from Bacteroides JY-6, an intestinal anaerobic bacterium of human. The specific activity of purified enzyme was 89.9 μmol/min/mg protein. The enzyme (M.W.240000) is composed of two subunits of M.W. 120000 with pI and optimal pH values of 4.2 and 7.0, respectively.The apparent K_ms for naringin, rutin and p-nitrophenyl-α-L-rhamnopyranoside were determined to be 0.89, 1.44 and 0.29 mM, respectively. The enzyme was strongly inhibited by L-rhamnose, L-fucose, saccharic acid 1, 4-lactone, p-chlormercuriphenylsulfonic acid and Pb²⁺.
关键词：α-rhamnosidase;intestinal bacteria;Bacteroides JY-6;purification