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  • 标题:MOLECULAR CLONING OF THE nemA GENE ENCODING N-ETHYLMALEIMIDE REDUCTASE FROM Escherichia coli
  • 本地全文:下载
  • 作者:Koichi MIURA ; Yoshihisa TOMIOKA ; Hideaki SUZUKI
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:1997
  • 卷号:20
  • 期号:1
  • 页码:110-112
  • DOI:10.1248/bpb.20.110
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:Using the gene mapping membrane technique, we identified a gene (nemA) that encodes N-ethylmaleimide reductace in Escherichia coli. The open reading frame encodes a polypeptide of 365 amino acids with a molecular mass of 39, 514 Da. The deduced amino acid sequence showed a high degree of homology (87% identical) with the pentaerythritol tetranitrate reductase of Enterobacter cloacae and the morphinone reductase of Pseudomonas putida (52% identical).
  • 关键词:Escherichia coli;N-ethylmaleimide reductace;unsaturated fatty acid;Old Yellow Enzyme
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