文章基本信息

标题：Effect of 15-Hydroperoxyeicosatetraenoic Acid on the Fibrinolytic Factor Release and the Antithrombin Binding of Vascular Endothelial Cells
本地全文：下载
作者：Shinji SOEDA ; Okinobu HONDA ; Nobuaki FUJII 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：1
页码：15-19
DOI：10.1248/bpb.20.15
出版社：The Pharmaceutical Society of Japan
摘要：15-Hydroperoxyeicosatetraenoic acid (15-HPETE), an arachidonate lipoxygenase product, is reported to induce severe endothelial injury. In this study, we examined the effect of 15-HPETE on the release of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) from cultured human umbilical vein endothelial cells (HUVEC). The addition of 15-HIPETE to the serum-free medium reduced the release of t-PA antigen from HUVEC, while the release of PAI-1 antigen was significantly enhanced. However, treatment of the cultured HUVEC with α-tocopherol or nordihyroguaiaretic acid completely suppressed the 15-HPETE-induced change in t-PA and PAI-1 antigen release. 15-Hydroxyeicosatetraenoic acid (15-HETE) had no effect on the release of either antigen from cultured HUVEC. The HUVEC surfaces exposed to 15-HPETE decreased the potency for binding antithrombin III. In a reconstituted system with heparin and phosphatidylcholine, 15-HPETE decreased the ability of heparin to inactivate thrombin activity. These results suggest that the fibrinolytic factor release and the antithrombin binding of vascular endothelial cells are impaired by the attack of 15-HPETE, and that the presence of antioxidants prevents the injurious action of lipid hydroperoxide.
关键词：endothelial cell injury;15-hydroperoxyeicosatetraenoic acid (15-HPETE);tissue-type plasminogen activator;plasminogen activator inhibitor-1;antithrombin binding