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标题：Simultaneous Determination of the Lactone and Carboxylate Forms of 7-Ethyl-10-hydroxycamptothecin (SN-38), the Active Metabolite of Irinotecan (CPT-11), in Rat Plasma by High Performance Liquid Chromatography
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作者：Norimasa KANEDA ; Yukiko HOSOKAWA ; Teruo YOKOKURA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：7
页码：815-819
DOI：10.1248/bpb.20.815
出版社：The Pharmaceutical Society of Japan
摘要：We established a high performance liquid chromatography (HPLC) method for the simultaneous determination of the lactone and carboxylate forms of 7-ethyl-10-hydroxycamptothecin (SN-38), the active metabolite of the antitumor drug irinotecan (CPT-11), in rat plasma. Plsma samples were pretreated with chilled MeOH and zinc sulfate to precipitate protein, and were then directly injected into the HPLC system. Chromatography was carried out with a Puresil C18 column (particle size 5 μm), and the mobile phase consisted of 0.1 M ammonium acetate buffer (pH 5.5) and acetonitrile (70/30, v/v) containing 20 mM of tetra-n-pentylammonium bromide. The column effluent was monitored with a spectrofluorometer (excitation wavelength 380 nm, emission wavelength 540 nm). The method was valid for SN-38 lactone (5-2500 ng/ml) and carboxylate (5-1000 ng/ml).
关键词：7-ethyl-10-hydroxycamptothecin (SN-38);lactone;carboxylate;simultaneous determination;irinotecan (CPT-11)