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标题：Effects of Various Absorption Enhancers on the Intestinal Absorption of Water Soluble Drugs by in Vitro Ussing Chamber Method : Correlation with an in Situ Absorption Experiment
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作者：Tetsuo SUGIYAMA ; Akira YAMAMOTO ; Yoshiko KAWABE 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：7
页码：812-814
DOI：10.1248/bpb.20.812
出版社：The Pharmaceutical Society of Japan
摘要：The effect of absorption enhancers on the small and large intestinal absorption of drug in rats was examined using an in vitro modified Ussing chamber method, and the results were compared with those from an in situ absorption experiment. Phenol red was chosen as a model drug, while the absorption enhancers used were sodium glycocholate (Na-GC), sodium taurocholate (Na-TC), sodium deoxycholate (Na-DC), EDTA, sodium salicylate (Na-Sal), sodium caprate (Na-Cap), diethyl maleate (DEM) and N-lauryl-β-D-maltopyranoside (LM), all used at a concentration of 20 mM. This modified Ussing chamber method showed that Na-DC and EDTA were the most effective absorption enhancers in the small intestine, whereas Na-DC, EDTA and LM were the most effective absorption enhancers in the large intestine. A good correlation exists between the area under the curve (AUC) (in situ loop model) and the cumulative amount of phenol red absorbed (in vitro medified Ussing chamber method). These results indicated that the in vitro modified Ussing chamber method can be used to evaluate the effects of various absorption enhancers in the intestine.
关键词：absorption enhancer;phenol red;intestinal permeability;in vitro modified Ussing chamber method