文章基本信息

标题：On the Antibacterial Activity of Normal and Reversed Magainin 2 Analogs against Helicobacter pylori
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作者：Akiyo IWAHORI ; Yukiko HIROTA ; Ruriko SAMPE 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：7
页码：805-808
DOI：10.1248/bpb.20.805
出版社：The Pharmaceutical Society of Japan
摘要：Magainin 2 is an antimicrobial peptide isolated from the skin of Xenopus laevis. We have tested the antibacterial activities of normal and reversed magainin 2 analogs against two strains of Helicobacter pylori (ATCC 43526, ATCC 43579), compared with those against Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923). Among these analogs, MSI-78A showed the strongest activity against H. pylori. The MIC (minimum inhibitory concentration) values were almost the same as those against E. coli and S. aureus. No or lesser activity was observed in all the reversed peptides compared to the corresponding normal magainin 2 analogs. Based on the CD (circular dichroism) measurement, the more active peptide tends to show a higher α-content. The positively-charged five amino acids (KILKK) positioned at the C terminus on the amphipathical α-helical structure play important roles in exerting the strong activity against H. pylori. This indicates that the net charge of the cell surface in H. pylori may be more negative than that of E. coli, though both strains belong to the same genus.
关键词：magainin 2 analogs;antibacterial activity;H. pylori;hemolysis