文章基本信息

标题：Analysis of the Distruption Mutant of the Oscillin Homolog Gene of Dictyostelium discoideum
本地全文：下载
作者：Yuki MATSUDA ; Yukito MASAMUNE ; Ken-ichi KODAIRA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：9
页码：915-919
DOI：10.1248/bpb.22.915
出版社：The Pharmaceutical Society of Japan
摘要：A homolog of oscillin, the Ca²⁺ oscillation-inducing factor of the hamster, was identified from the cellular slime mold Dictyostelium discoideum and designated Dd-oscillin. In the developmental stages of D. discoideum, the gene is expressed at the prestalk region which contains higher concentration of cytosolic Ca²⁺ than the prespore region. The Dd-oscillin null strain aggregated but did not develop further when the cells were plated on non-nutrient agar at a density of 1.5×10⁶ cells/cm², showing that the Dd-oscillin gene is important for development. Since the null cells carrying the hamster oscillin gene formed fruiting body, the hamster oscillin was the homolog of Dd-oscillin as far as function is concerned. In addition, the null cells formed fruiting body in the presence of 2, 5-di(tert-butyl)-1, 4-hydroquinone (BHQ : a specific inhibitor of Ca²⁺-ATPase activity in the endoplasmic reticulum). These results suggest that Dd-oscillin will increase cytosolic Ca²⁺ in the cells and promote further development.
关键词：Dictyostelium discoideum;calcium ion oscillation;oscillin