文章基本信息

标题：Identification of a High-Copy-Number Plasmid Suppressor of a Lethal Phenotype Caused by Mutant DnaA Protein Which Has Decreased Instrinsic ATPase Activity
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作者：Masaki MAKISE ; Kenji SAKAMOTO ; Tomofusa TSUCHIYA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：9
页码：904-909
DOI：10.1248/bpb.22.904
出版社：The Pharmaceutical Society of Japan
摘要：The induction of a mutant DnaA protein (DnaA E204Q) with decreased intrinsic ATPase activity in cells causes a lethal phenotype. Based on our results that the decreased ATPase activity of DnaA E204Q is activated to the level of a wild-type protein in the presence of partially purified stimulating factors for DnaA ATPase, we tested the hypothesis that genes encoding the stimulating factors can be cloned as high-copy-number plasmid suppressors for the lethality caused by DnaA E204Q. We isolated a number of high copy-number plasmids that suppress the lethal phenotype. The genes responsible for the suppression of the lethal phenotype were revealed to be dnaN, relA, pcnB and cyaA by subcloning, and a site-directed mutational analysis. The mechanisms by which these genes suppressed the lethal phenotype were examined in vivo and in vitro.
关键词：DNA replication;DnaA;the dnaN gene;ATPase;suppressor