文章基本信息

标题：In Vitro Transcriptional Analysis of the Cytochrome P-450cam Hydroxylase Operon
本地全文：下载
作者：Hironori ARAMAKI ; Yasuhiro SAGARA ; Masaya FUJITA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：10
页码：1110-1112
DOI：10.1248/bpb.22.1110
出版社：The Pharmaceutical Society of Japan
摘要：To characterize the promoters of cytochrome P-450cam hydroxylase operon (camDCAB) and the repressor gene (camR), in vitro run-off transcription assays were performed using RNA polymerase (RNAP) holoenzyme reconstituted with the core enzyme and the σ⁷⁰ protein of Pseudomonas aeruginosa. Both the mRNAs of camDCAB and camR were accurately transcribed from the respective promoter by the reconstituted RNAP holoenzyme. Both the transcriptions were repressed by CamR protein and the repressions were suppressed by D-cam-phor, consistent with the regulation in P. putida. These results suggest that the RNA polymerase containing σ⁷⁰ recognizes the promoter of camDCAB as well as that of camR.
关键词：cytochrome P-450cam hydroxylase operon;Pseudomonas putida;RNA polymerase;σ<70>transcription