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标题：Bactericidal Activity of Lemon Juice and Lemon Derivatives against Vibrio Cholerae
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作者：Marta Cecilia DE CASTILLO ; Cristina Gaudioso DE ALLORI ; Rosa Cangemi DE GUTIERREZ 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2000
卷号：23
期号：10
页码：1235-1238
DOI：10.1248/bpb.23.1235
出版社：The Pharmaceutical Society of Japan
摘要：Food products can be possible vectors of the agent responsible for cholera epidemics, because some of these products allow Vibrio cholerae O1 to develop to concentrations above the dangerous level. This study deals with the behaviour of essential oils, natural and concentrated lemon juice and fresh and dehydrated lemon peel against V. cholerae O1 biotype Eltor serotype Inaba tox⁺. Our aim was to evaluate whether these products, used at different dilutions, exhibit bactericidal or bacteriostatic activity against the microorganism, when present at concentrations of 10², 10⁴, 10⁶ and 10⁸ colony forming units (CFU) ml^-1, and after different exposure times. 10⁸ CFU ml^-1 was considered an infectious dose. Concentrated lemon juice and essential oils inhibited V. cholerae completely at all stuidied dilutions and exposure times. Fresh lemon peel and dehydrated lemon peel partially inhibited growth of V. cholerae. Freshly squeezed lemon juice, diluted to 10^-2, showed complete inhibition of V. cholerae at a concentration of 10⁸ CFU ml^-1 after 5 min of exposure time; a dilution of 2×10^-3 produced inhibition after 15 min and a dilution of 10^-3 after 30 min. It can be concluded that lemon, a natural product which is easily obtained, acts as a biocide against V. cholerae, and is, therefore, an efficient decontaminant, harmless to humans.
关键词：Vibrio cholerae;lemon product;bactericide;bacteriostatic