文章基本信息

标题：Molecular and Functional Analysis of Cellular Phenomena Using Single-Cell Mass Spectrometry
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作者：Naohiro Tsuyama ; Hajime Mizuno ; Tsutomu Masujima 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2012
卷号：35
期号：9
页码：1425-1431
DOI：10.1248/bpb.b212012
出版社：The Pharmaceutical Society of Japan
摘要：Single-cell analysis has attracted attention in many fields of biological studies as a tool to survey the precise mechanisms of cellular and molecular behavior. The development of sensitive mass spectrometry allows the study of molecules in single cells or small regions. Matrix-assisted laser desorption/ionization imaging mass spectrometry and secondary-ion mass spectrometry use in situ ionization of specimens on sample plates to visualize molecular distributions as images from mass spectra. Several single-cell mass spectrometry technologies that initially recover a single cell followed by ionization have been developed. Among them, only nanospray-mediated sampling and ionization named Live Single-cell Mass Spectrometry can be used for real-time analysis. This paper explains that method in detail.
关键词：single-cell mass spectrometry;cell heterogeneity;metabolome;drug metabolism