文章基本信息

标题：Proteomic approaches to profiling the humoral immune response and identifying disease-Associated antigens
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作者：Kaname Ohyama ; Naotaka Kuroda
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2012
卷号：35
期号：9
页码：1409-1412
DOI：10.1248/bpb.b212010
出版社：The Pharmaceutical Society of Japan
摘要：In many diseases ( e.g. autoimmune diseases, cancer), a humoral immune response is thought to be involved; therefore, identifying relevant antigens that trigger the humoral immune response is at the heart of current approaches for discovering biomarkers and designing therapeutic strategies. Although immunoblotting and protein array have been used for antigen profiling in several diseases, with the rapid progress in mass spectrometry (MS) and separation technologies, MS-based proteomic methods have become a promising tool for identifying disease-associated antigens. This review introduces MS-based antigen profiling methods (immunoproteomics and immune complexome analysis) and presents application examples of these techniques to the analysis of biological samples.
关键词：mass spectrometry;immune complexome analysis;antigen profiling;immunoproteomics