文章基本信息

标题：牛肉中のジエチルスチルベストロールグルクロン酸抱合体のガスクロマトグラフィーによる定量
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作者：飯島正雄 ; 星野庸二 ; 能勢憲英等
期刊名称：Journal of Health Science
印刷版ISSN：1344-9702
电子版ISSN：1347-5207
出版年度：1993
卷号：39
期号：4
页码：311-316
DOI：10.1248/jhs1956.39.4_311
出版社：The Pharmaceutical Society of Japan
摘要：A gas-liquid chromatographic (GLC) method was developed for the determination of diethylstilbestrol glucuronide (DESG), a metabolite of diethylstilbestrol, in beef. DESG was extracted from beef with 90% acetonitrile. The extract was cleaned up without hydrolysis of DESG by Sep-pak NH₂ cartridge and C₁₈ cartridge. The purified extract was evaporated to dryness and reacted with pentafluoropropionic acid anhydride (PFPA) for 50 min at 50°C. PFPA derivatives of DESG were measured by GLC, using a 2% OV-17 column and electron capture detector. Recoveries of DESG obtained from beef spiked at 0.005-0.02 μg/g (as is DES) levels were 86.8-89.2%. The detection limit of DESG was 0.001 μg/g.
关键词：diethylstilbestrol glucuronide;GLC;pentafluoropropionic acid anhydride, beef