文章基本信息

标题：ISOLATION OF LYSOZYME FROM URINE OF PATIENTS WITH ITAI-ITAI DISEASE
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作者：Masami KIMURA ; Noriko OTAKI
期刊名称：Industrial Health
印刷版ISSN：0019-8366
电子版ISSN：1880-8026
出版年度：1978
卷号：16
期号：3-4
页码：127-137
DOI：10.2486/indhealth.16.127
出版社：National Institute of Occupational Safety and Health
摘要：Elevated lysozyme activity was observed in the urine of patients with Itai-itai disease. The urinary lysozyme was isolated from the urine specimens of these patients by gel filtration and ion exchange chromatography. Two lysozyme peaks were found on Sephadex G-100 gel filtration. The first peak (lysozyme-E) had two components on DEAE-cellulose chromatography. One of them showed more acidic chromatographic behavior than the other component which was eluted at the void volume of the column. The second peak (lysozyme-F) on Sephadex G-100 gel filtra-tion had only the component which was similarly eluted at the void volume of the DEAE-cellulose chromatographic column. Slight differences in the chromatographic behavior and in amino acid composition between lysozyme-E and -F indicated that the urinary lysozyme was composed of two components.