文章基本信息

标题：Separation of Major Mononitro-aromatic Compounds by Reversed-phase High-performance Liquid Chromatography
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作者：Masahiro YOSHIKAWA ; Junko BESSHO ; Keiichi ARASHIDANI 等
期刊名称：Industrial Health
印刷版ISSN：0019-8366
电子版ISSN：1880-8026
出版年度：1996
卷号：34
期号：3
页码：177-183
DOI：10.2486/indhealth.34.177
出版社：National Institute of Occupational Safety and Health
摘要：A simple analytical method was developed to determine p -nitrochlorobenzene ( p -NCB) in working environmental air by reversed-phase high-performance liquid chromatography (HPLC). Nitrobenzene, nitrotoluene isomers, and NCB isomers were separated and p -NCB was determined by reversed-phase HPLC as follows: column; Hitachi gel #3056 (4.0mm I.D. x 150mm), mobile phase; 2-propanol: water (40:60, v/ v) with a flow rate of 0.8ml/min, column temperature; 50°C, detector; UV detector at 270nm. Nitrobenzene, o -, p -nitrotoluene, and o -, m -NCB were separated from p -NCB, but m -nitrotoluene could not be completely separated from p -NCB. The limit of detection (signal-to-noise ratio of 3:1) of p -NCB was 0.3ng. Repeatability of the HPLC method was excellent. The HPLC method enabled to determine one-tenth of the administrative level (1mg/m³) of p -NCB, since the lower limit of determination was 0.05mg/m³. Therefore, the present HPLC method proved to be applicable to the determination of p -NCB in working environmental air.
关键词：High-performance liquid chromatography;p-Nitrochlorobenzene;Nitrobenzene